TARDBP and amyotrophic lateral sclerosis: In order to determine if ALS-causing mutations of TDP-43 enhance the association with SGs in motoneuron-like cells, we first stably transfected NSC-34 cells with TDP-43WT, fALS-causing mutations TDP-43A315T, and TDP-43M337V, that were N-terminally tagged with Enhanced Green Fluorescent Protein (EGFP), or transfected with EGFP alone.