It is possible that our ALS-linked TDP-43 mutations, under osmotic stress, alter the nature of TDP-43 interaction with proteins such as FUS and SG proteins G3BP and TIA-1 (McDonald et al., 2011; Baron et al., 2013; Sama et al., 2013; Lenzi et al., 2015; Markmiller et al., 2018) to maintain the presence of mutated TDP-43-containing SGs long after removal of stress. This evidence concerns the gene TIA1 and amyotrophic lateral sclerosis.