With respect to the above, the present study was conducted focusing on the capture of CTCs in peripheral blood of healthy subjects, patients with high-risk population (HRP) and those with different types of cancer on the basis of the preparation of EpCAM+Vimentin specific immunomagnetic beads, with the aim to study the difference of CTCs counts of non-cancer patients with healthy subjects and cancer patients. This evidence concerns the gene EPCAM and cancer.