For instance, we have engineered ATRX-deficient gliomas (148, 149), by injecting plasmids encoding SB transposase/firefly luciferase, plus other plasmids encoding for the desired genetic alterations located between IR/DR: shp53, NRASG12V, and shATRX, into the lateral ventricle of neonatal mice (148). Here, ATRX is linked to central nervous system cancer.