In this study, Nile red staining showed that a large number of lipid droplets were formed during the late stages of CF (S3 and S4) (Figure 4), and genes related to fatty acid metabolism were indeed differentially expressed (Supplementary Table 23), including genes encoding acetyl-CoA C-acetyltransferase (TRIVIDRAFT_169943), enoyl-CoA hydratase (TRIVIDRAFT_87842), acetyl-CoA carboxylase (TRIVIDRAFT_78374), fatty acid synthase subunit beta, fungi type (TRIVIDRAFT_171412), fatty acid elongase 3 (TRIVIDRAFT_82162) and acetyl-CoA acyltransferase 1 (TRIVIDRAFT_80821). Here, ACAA1 is linked to cystic fibrosis.