To understand such mechanisms, we used RNA-seq to determine the transcriptomics of CF in T. virens GV29-8 at different developmental stages, hoping to reveal transcriptome-based dynamics of CF, and identify key determining factors of CF in T. virens. The transcript modules of co-expressed genes at different stages of CF and the series test of cluster (STC) analysis of differential time points during gene expression were performed to identify candidate genes and pathways that might determine CF. Here, CFTR is linked to cystic fibrosis.