To investigate the effect of TGF-β-induced EMT on metabolism in NSCLC cells, three well-studied NSCLC cell lines (A549, HCC827, and H358) were treated with TGF-β for 3 days as well as several weeks, and polar metabolites were quantified by capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS)24 (Supplementary Data 1–3). The gene discussed is TGFB1; the disease is non-small cell lung carcinoma.