To further determine whether the effect of DT-mediated depletion of macrophages on reducing liver fibrosis is attributed to inhibition of H19, adeno-associated virus serotype 9 (AAV9) overexpressing H19 (AAV9-H19) was used to overexpress H19 in CD11b-DTR mice via tail vein injection. The gene discussed is ITGAM; the disease is Hepatic fibrosis.