In C57BL/6J mice, both real-time quantitative PCR and western-blot analysis showed that expression of TLR4, MyD88, TRAF6, IKKβ, RIP1, IRF3, and NF-κB p65 were significantly increased in HF than those in NC (Fig. 2A, B). This evidence concerns the gene TRAF6 and hydrops fetalis.