DICER1 and keratoconus: However, in this study the authors transfected miRNA-coding plasmids as opposed to mature miRNA.[19] This might not be as useful in certain diseases like diabetes, where components of the machinery responsible for miRNA processing, such as Dicer, are impaired.[34] This deficiency may impact the ability to process the miRNA gene-encoding plasmids efficiently, while also being limited for cell types where liposomal-based transfection is inefficient, such as primary KC.