LncRNA USP30−AS1 had co-expression relationship with 9 ferroptosis-related genes (CYBB, SOCS1, IFNG, TNFAIP3, PML, GCH1, NCF2, SLC2A6, and CAPG), including 4 ferroptosis driver, 2 ferroptosis suppressor and 3 ferroptosis marker, and upregulation of USP30−AS1 in breast cancer tissue was associated with longer overall survival time in this study. The gene discussed is CYBB; the disease is breast cancer.