Metformin also inhibits ROS accumulation and NLRP3 inflammasome activation stimulated by high glucose in macrophages which is blocked by compound C. These results indicate that metformin can inhibit NLRP3 inflammasome activation in apoE-/- mice and inhibit diabetes-accelerated AS, at least in part by activating AMPK and regulating thioredoxin-1/thioredoxin interaction protein (70). Here, APOE is linked to diabetes mellitus.