PFKFB3 and type 1 diabetes mellitus: To determine the effect glycolysis inhibition would have on the activation and subsequent metabolic reprogramming of autoreactive CD4+ T cells in T1D, we stimulated splenocytes from NOD.BDC2.5.TCR.Tg animals in vitro with their cognate peptide mimotope (MM) ± PFK15 (a PFKFB3 inhibitor; Figure 1A), as previously described (18).