To investigated whether sirtuins affect stem-like phenotypes in pancreatic cancer cells, FLAG-tagged SIRT1–7 were stably expressed in PANC-1 cells; using flow cytometry, CD133+ cell content increased in cells overexpressing SIRT1 (Fig. 1B); there was no significant change in other cells. This evidence concerns the gene PROM1 and familial pancreatic carcinoma.