In total mRNA expression of TIMP-1, ANGPT1, SPP1, TGF-β1 and YKL-40 genes was analysed in 61 patients with different grade astrocytoma: 20 grade II diffuse astrocytoma, and 41 grade IV astrocytoma (glioblastoma), and in case of IP-10—in 11 grade II diffuse astrocytoma, and 24 glioblastoma samples. The gene discussed is SPP1; the disease is glioblastoma.