Earlier we demonstrated5 decision tree which consisted of ANGPT-1, TIMP-1, IP-10 and active TGF-β1 proteins, algorithm reached accuracy of 73.5% (75/102) and correctly classified 79.7% (47/59) of all astrocytomas and 65.1% (28/43) of all healthy controls. Here, TGFB1 is linked to astrocytoma (excluding glioblastoma).