Thus, we reasoned that a subgroup of genes induced by TSA would be transactivated in a BRD4‐dependent manner that relies on acetylated H3, resulting in the activation of ERK1/2 in ESCC cells and we found that TSA increased the expression of BRD4 in ESCC cells (Figure 5A). This evidence concerns the gene BRD4 and esophageal squamous cell carcinoma.