As a result, LAG3, NRP1, NT5E, CCL21, CCR7, and tumor necrosis factor receptor super family (TNFRSF) including TNFRSF 13B, TNFRSF17, TNFRSF9, TNFRSF8, TNFRSF13C, TNFRSF11B, and so on, were found significantly overexpressed in metastatic melanoma, and may involve autoimmune suppression in the tumor microenvironment and induce tumor evasion. The gene discussed is NT5E; the disease is metastatic melanoma.