To investigate the effects of MDP neoantigen on the anti-tumor immune responses, we firstly measured the proportion of infiltration of T cells in the 4T1 breast cancer allografts by IHC staining for CD8 and found the significantly increased CD8+ T cell infiltration in the tumor allografts treated with AC4ManNAZ and DP (Figure 5A). Here, CD8A is linked to neoplasm.