Next, WB and IF assays were applied to confirm that MRPL52 increased the expression of N-cadherin, and Vimentin (mesenchymal markers), and decreased the expression of E-cadherin and ZO-1 (epithelial markers) via provoking Snail expression in hypoxic BC cells (Figure 5E-F). The gene discussed is TJP1; the disease is breast cancer.