We, therefore, investigated the potential biological processes associated with immune cytolytic activity (CYT) which was used to assessed immune evasion using the geometric mean of GZMA and PRF1 expression values.30 By dividing tumors into CYThigh and CYTlow groups for each cancer type, the results of GSEA revealed that immune-related pathways and EMT processes were significantly enriched in CYThigh tumors (Fig. 1B and Supplementary Fig. 1B). Here, PRF1 is linked to cancer.