Furthermore, there is still a considerable number of CAR T cells close to the residual tumor surface that does not express IFNγ or GZMB. A potential explanation for this may be the expression kinetics of IFNγ or GZMB post-CAR T cell activation and the relatively late time point of tumor collection post-CAR T cell infusion, hence, some CAR T cells may have experienced antigen exposure early after infusion but may have not been stimulated a second time. This evidence concerns the gene GZMB and neoplasm.