Consistent with the results obtained from TCGA data analyses, an inverse correlation between METTL3 protein expression and APC protein expression (Fig. 7c, d), and a positive correlation between METTL3 protein expression and β-catenin protein expression (Fig. 7c, e) were observed by IHC staining of 81 ESCC specimens. This evidence concerns the gene APC and esophageal squamous cell carcinoma.