APCDD1 and neoplasm: The transcriptional profiling of PDCOs confirmed the differential expression of CRC markers (e.g. MUC1, MUC4 and CA2 up-regulated in normal organoids; PROX1, BAMBI, PTCH1 and APCDD1 up-regulated in tumor organoids) [18] but applying the likelihood ratio test also allowed to study combined changes induced by time and condition, finding for instance that the APCDD1 up-regulation, compared to normal organoids, is progressively reduced during tumor PDCOs development.