Considering growth medium conditions, in a couple of comparisons (“P12T -W early_P12T primary tissue” and “P14T -W late_P14T primary tissue”) we observed variants in the PTEN and RET genes that were not present in the corresponding T + W early sample; however, when directly comparing T -W early and T + W early samples, these variants disappeared indicating similar AF. Here, PTEN is linked to atrial fibrillation.