Pie-charts indicate the proportion of different macrophage populations and demonstrate that the major shift in macrophage phenotype in the hypothalamus in obesity was an increase of the PDL1+ M1 population (from 39 to 71%) and a decrease of the CD206+ M2 population (from 33 to 4%; Fig. 4A). This evidence concerns the gene MRC1 and obesity due to melanocortin 4 receptor deficiency.