The present study determined the differential expression status of mutant genes in carcinoma and para‐carcinoma tissues from 199 patients with EJA using next‐generation sequencing (NGS) and preliminarily screened lymph node metastasis‐associated genes, including CDK6, MET, and NOTCH1, as well as low‐density lipoprotein receptor‐related protein 1B (LRP1B). This evidence concerns the gene LRP1B and carcinoma.