Interestingly, IFI16 overexpression specifically increased the population of CD11b + F4/80 + macrophages in the tumor microenvironment but had minimal effect on the populations of CD11b + CD11c + dendritic cells, CD3 + CD4 + CD8- T helper cells, and CD3 + CD4-CD8 + cytotoxic T cells, suggesting that the effect of IFI16 overexpression in PAAD cells may specifically target TAMs in the tumor microenvironment (Figure 2D, gating shown in Supplementary Figure 1). This evidence concerns the gene ITGAX and pancreatic adenocarcinoma.