To further understand how inflammasome activation and IL-1β production by IFI16-overexpressing PAAD cells regulate TAMs, we used neutralizing antibodies to block IL-1β in the culture supernatant from wild-type and IFI16-overexpressing PAAD cells, according to a published protocol with minor modifications (Staudt et al., 2013). This evidence concerns the gene IL1B and pancreatic adenocarcinoma.