Among the three main types of employed protocols, the EB-FHP+MY type seems to have the best balance between the reproducibility (due to the factor-mediated control of the M/HE stage), clinical applicability (due to the use of xeno-free conditions and defined medium), efficacy (due to the continuous generation of iMphs), and cost (due to the use of only two cytokines, IL-3 and M-CSF, for HP/MY differentiation). The gene discussed is IL3; the disease is hereditary elliptocytosis.