HES1 and cleft palate: Our data show that signaling pathways such as Wnt (Wnt5a, Axin1, Axin2), Fgf (Etv4, Etv5, Spry1), Shh (Gli, Shh), Bmp (Bmp2, Bmp4), or TGF-β (Tgf-β) or Notch (Hes1, Notch2) are not significantly affected by Wiz ablation at this stage as well as expression of other cleft palate associated genes like Msx2 or Cdh1.