To assess the function of BDH1 gene in AML, we first cloned the Bdh1 CDS into MSCV-PIG retroviral vector, and then co-infected mouse bone marrow (BM) progenitor cells with MSCVneo-MLL-AF9 (MA9) and MSCV-PIG-Bdh1 (Bdh1) or MSCV-PIG (Ctrl) retroviruses. Here, KMT2A is linked to acute myeloid leukemia.