Five metabolites including uracil, arginine, lactate, cystamine, and ornithine, significantly differed between high and low grade glioma patientsUridine (P = 3.76 × 10−4, q = 0.015) and ornithine (P = 9.36 × 10−4, q = 0.038) were identified which differed between GBM and malignant glioma patientsN-acetylputrescine (P = 9.12 × 10−4, q = 0.036) and trimethylamine-N-oxide (TMAO) (P = 0.006, q = 0.043) were identified which differed between IDH mutation positive and negative tumors. The gene discussed is IDH1; the disease is malignant glioma.