Afterward, in-depth experimental research detected more star mechanisms (such as GPX4, Nrf2, GSH, p53, SLC7A11, ACSL4, system xc−, phospholipids, NAD(P)H, CoQ10, lipid peroxidation, etc.), used new technologies, and related ferroptosis to more diseases (such as kinds of cancer, Alzheimer, Parkinson’s disease, stroke, ischemia-reperfusion damage, kidney disease, liver disease, atherosclerosis, drug resistance, etc.). This evidence concerns the gene ACSL4 and Stroke.