To evaluate the expression of GPC-3 in different cancer, all cancer tissues were immunohistochemically (IHC) stained with anti-GPC-3 antibody (Abcam, Cambridge, MA, USA, RRID: AB_2476152) and visualized by an ABC peroxidase standard staining kit (Thermo Scientific, Waltham, MA, USA). The gene discussed is GPC3; the disease is cancer.