After 6 h, the total protein was extracted for phosphorylation protein microarray analysis, which found that AKT and GSK3β protein phosphorylation ratios in the sh-cancer-IgG group were reduced and that Bcl-2, BAD, caspase 3, caspase 9, and BAX apoptosis-related proteins increased (Figure 5A). Here, CASP3 is linked to cancer.