Moreover, we demonstrated that PPP1R12A, a subunit of myosin phosphatase that play an critical role in regulating cell cycle and migration 25, 34, 39, was a new direct target of miR-455-5p and PPP1R12A expression was negatively correlated with miR-455-5p in CCA tissues. Here, PPP1R12A is linked to cholangiocarcinoma.