In addition, we checked signalling molecules downstream of β-catenin and found that TCF-4, c-Myc and Cyclin D1 were reduced in Mist1-overexpressing cells, which indicates that the Wnt/β-catenin signalling pathway was inhibited in Mist1-overexpressing gastric cancer cells (Fig. 4D, E). This evidence concerns the gene BHLHA15 and gastric cancer.