To confirm the expression of Mist1 in gastric cancer cells, we assessed seven gastric cell lines by qPCR and Western blotting, which indicated the downregulation of Mist1 expression in gastric cancer cells compared with SW620 cells, consistent with the changes in Mist1 expression in gastric cancer tissues (Fig. 1G, H). The gene discussed is BHLHA15; the disease is gastric cancer.