Therefore, we next analyzed the effects of SHMT2 in BLCA cell cycle, and the results demonstrated that overexpression of SHMT2 promote G1/S phase transition, silencing of SHMT2 significantly inhibited cell cycle G0/G1 phase to S phase transition by downregulating cyclin E1 and cyclin D1 protein expression. Here, CCNE1 is linked to bladder transitional cell carcinoma.