The results showed that SHMT2 expression was significantly higher in patients older than 60 years (P = 0.024) (Figure 4A), with a high tumor grade (P = 0.0003 < 0.001) (Figure 4B), and in dead BLCA patients (P = 0.049) (Figure 4C), but showed no association with tumor (T) stage (P = 0.84), node (N) stage (P = 0.43), metastasis (M) stage (P = 0.45), TNM stage (P = 0.84) and gender (P = 0.73) (Figures 4D–H). The gene discussed is SHMT2; the disease is bladder transitional cell carcinoma.