Next, GSE3167 (nine normal bladder samples versus 51 BLCA samples, P < 0.0001), GSE7476 (three normal bladder samples versus nine BLCA samples, P < 0.05) (Figure 2B), and TCGA RNA-Seq expression data (19 normal bladder tissues versus 414 BLCA tissues, P = 9.61E-11; 19 BLCA and 19 matched normal bladder tissues, P = 6.86E-08) were selected to verify that SHMT2 was overexpressed in BLCA tissues (Figure 2C). Here, SHMT2 is linked to bladder transitional cell carcinoma.