As shown in the Figure 4D, the top three proteins (CKAP5, AURKA, and CDC20) interacting with TACC3 were verified by immunoprecipitation in breast cancer cells, and CKAP5, AURKA, and CDC20 were detected in the immunoprecipitation of anti TACC3 antibody. This evidence concerns the gene CKAP5 and breast carcinoma.