CD4 and neoplasm: Interestingly, at 8 days post cryoablation, frequencies of CD4+ conventional T-cells and their proliferation status were significantly increased in both the TDLN and the contralateral tumor non-draining lymph node (TNDLN) only in the fast freeze group (Figures 3E, F and Supplementary Tables 2, 4), indicative of systemic immune responses.