Of fifteen DEGs associated with and regulated by Sirtuin signaling pathway in our study (Supplementary Table 8), ATG4D (Autophagy Related 4D Cysteine Peptidase), a regulator of autophagy which is a mechanism that protect cells from degradation under stress conditions such as energy deprivation, and virus infection, was significantly upregulated after CSBV infection. The gene discussed is ATG4D; the disease is viral infectious disease.