More recently, it appears that ALS pathology involves more than the motor system and pathological TAR DNA-binding protein 43 (TDP-43) inclusions have been confirmed to be in four stages in the whole brain, inclusive of extra-motor cortical and subcortical structures and the brainstem (Braak et al., 2013; Brettschneider et al., 2013; Qiao et al., 2020). This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.