In our study, we discovered that PCGF1 upregulated the expression of stemness markers by maintaining the histone H3K4me3 status by increasing the expression of KMT2A and removing the histone H3K27me3 status by increasing the expression of KDM6A, suggesting that KDM6A and KMT2A play a vital role in colorectal cancer stem cells. This evidence concerns the gene KDM6A and colorectal cancer.