Our data indicate that loss of PCGF1 decreases H3K4me3 and increases H3K27me3 at the promoters of stemness markers, suggesting that PCGF1 activates the stemness markers CD133 and CD44 through the H3K4me3 and H3K27me3 modifications in the promoter region of colorectal cancer stem cells. Here, CD44 is linked to colorectal cancer.