When TMX was injected at 4 weeks old, eR1-CreERT2 Tg; Rosa26-LSL-RUNX1-ETO-IRES-EGFP; Rosa26-LSL-NrasG12D mice developed leukemia with complete penetrance and shorter latency as compared to RUNX1-ETO alone model (Fig. S5). The gene discussed is RUNX1T1; the disease is leukemia.