To exclude the possibility of silencing of GFP expression in the MPD cases, namely the residual RUNX1-ETO expression in the GFP- fraction, semi-quantitative polymerase chain reaction (PCR) and quantitative reverse transcription-PCR (qRT-PCR) were conducted on genomic DNAs and RNAs extracted from 6 MPD cases, respectively (Fig. S3B, C). This evidence concerns the gene RUNX1 and myeloproliferative disorder.