RUNX1T1 and leukemia: Lastly, qRT-PCR and western blot analyses demonstrated that the expression level of RUNX1-ETO mRNA and protein in this mouse model is comparable to that in t(8;21) clinical samples, and not as high as those in RUNX-ETO expressing human leukemia cell lines, Kasumi-1 and SKNO-1, and a mouse cell line retrovirally transduced with RUNX-ETO9a (Fig. 2F, G).