The purpose of the study was to evaluate the diagnostic value of gamma‐glutamyl transpeptidase to alkaline phosphatase ratio (GAPR) combined with gamma‐glutamyl transpeptidase to aspartate aminotransferase ratio (GAR) and alanine aminotransferase to aspartate aminotransferase ratio (AAR) in alpha‐fetoprotein (AFP)‐negative hepatocellular carcinoma (HCC). The gene discussed is GPT; the disease is hepatocellular carcinoma.