We confirmed the cancer-associated upregulation of PLANE in cohorts of formalin-fixed paraffin-embedded (FFPE) LUSC, LUAD and COAD and freshly isolated ESCC and BRCA samples compared to adjacent normal tissues (Fig. 1a and Supplementary Fig. 2a). This evidence concerns the gene MELTF-AS1 and esophageal squamous cell carcinoma.