To explore the possible underlying mechanisms of the Irisin/Integrin αV complex during osteogenic and adipogenic differentiation and answer the question of whether Irisin is an upstream regulator of SMAD1/5, we transfected BM-MSCs which were maintained in OM for 14 days with pcDNA3.1 vector encoding Irisin. This evidence concerns the gene FNDC5 and ocular melanoma.