These genes include known LKB1 target genes, such as INSL4, CPS1, NR4A2, LINC00473, NR4A1, PTGS2, SIK1, and PDE4D. We also validated the downregulation of ID1 in dnCRTC-expressing A549 cells (Figure 4—figure supplement 1), a CRTC2/CREB target recently reported to be important in regulating LKB1-deficient lung cancer (Rodón et al., 2019), although it had a fold change of −1.72 (FDR p<0.05) in dnCRTC-expressing vs. control A549 cells in our profiling experiment. This evidence concerns the gene PDE4D and lung cancer.