For subcutaneous xenograft models, luciferase-expressing LKB1-inactivated lung cancer cells (A549-luc and H157-luc) were transduced with retroviruses expressing dnCRTC or control GFP for 72 hr, and then dnCRTC or GFP-transduced cells (106 cells per mouse) were subcutaneously implanted into immunodeficient NOD/SCID mice. The gene discussed is STK11; the disease is lung carcinoma.