INS and obesity due to melanocortin 4 receptor deficiency: Therefore, identifying an easy, reliable and cost-effective tool for the stratification of cardio-metabolic risk in youths with body weight excess is a primary goal to achieve for containing the burden of T2D and other metabolic complications of obesity across the new generations[43].Indeed, although the euglycaemic-hyperinsulinemic clamp represents the “gold standard” for measuring insulin sensitivity[44], this technique is invasive, expensive and difficult to perform in the clinical practice.