Macrophages in atherosclerosis arise from both migration from the bone marrow via peripheral blood and proliferation of resident macrophages.20 In addition, previous studies demonstrated that foam cells expressing p16-3MR can be removed from atherosclerotic plaques by GCV.5 We therefore isolated bone marrow-derived macrophages (BMDMs) from p16-3MR mice and cultured them for 1 day, 7 days, 21 days or 28 days to allow differentiation. Here, CDKN2A is linked to atherosclerosis.