Measurement of the concentrations of serum pro- and anti-inflammatory cytokines (IL-1β, IFN-γ, TNF-ɑ and IL-10) quantified for each mouse before infection, after infection and after pyocin injection showed there was no significant difference in cytokine levels following treatment of infected mice with BSA, pyocin S5 or pyocin AP41 (Figure S3). Here, IFNG is linked to infection.