Flow cytometry analysis of TAMs, regulatory T cells (Tregs), and myeloid-derived suppressor cells (MDSCs) with immune-suppressive capabilities isolated from subcutaneous tumors indicated that the percentages of total F4/80+ CD11b+ TAMs in CD45+ tumor-infiltrating leukocytes (TILs) and CD206+ F4/80+ CD11b+ macrophages (M2 macrophages) in total TAMs were both markedly increased in Spib-expressing tumors (Figures 2A, B and S2A). This evidence concerns the gene MRC1 and neoplasm.