However, the D‐loop methylation level was only correlated with BMI, which was in line with the recent study.[43] Although the lack of human hepatic ND6 expression pattern in T2DM population presents a limitation of the study, we have substantial evidence to demonstrate that instead of obesity itself, a direct link lies between elevated free fatty acid and hepatic insulin resistance, which we suggest playing a vital role during the onset of high calorie high fat associated insulin resistance. Here, MT-ND6 is linked to Insulin resistance.