In addition, the ex vivo methylation changes of NF-κB p65 in endothelial cells have also been shown in blood mononuclear cells of T2DM patients, where the unbalance is also related to an increased expression of MCP-1, intercellular adhesion molecule (ICAM)-1 and COX-2 (49). This evidence concerns the gene PTGS2 and type 2 diabetes mellitus.